Informatics Publishing Limited

Mohamad Azhar A. Patwekar ¹, V. R. Salunkhe ²

Affiliations
1 Rajarambapu College of Pharmacy, Kasegaon-415404, Maharashtra, IN
2 Rajarambapu College of Pharmacy, Kasegaon, Dist:Sangli (M.S), IN

Abstract

Derivatization is the process by which a compound is chemically changed, producing a new compound that has properties more amenable to a particular analytical method. Derivatization spectroscopy can be used for preparation of new derivatives from original one which can be detect in UV region. Several methods like nitration, sulphonation, methylation, esterification, acetylation and diazotization has been used for formation of new derivative which may be color or colorless and can be detected in UV region. Derivatization is an integral part of chemical analysis in many areas of chemistry such as medical, forensic, food science, and environmental disciplines. Derivatization enables the analyst to analyze a wide variety of compounds by GC, GC-MS, HPLC and LC-MS that were otherwise less volatile and unstable for these techniques. In general, analytical derivatization is employed for two reasons, to permit analysis of compounds with inadequate volatility or stability and to improve chromatographic behavior or detectability. The primary use of derivatization is the chemical modification of a compound by derivatizing its functional group (e.g., O-H, COOH, N-H, and S-H) to promote the use of chromatographic analysis. The underivatized compounds can demonstrate poor chromatographic behavior, insufficient volatility, and poor thermal stability or have inadequate detector response. By chemically modifying these compounds into derivatives they have properties amendable for chromatographic separation and accurate analysis.

Keywords

Derivatization, Need, Types, Derivatization Methods, Example of Derivatization.

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Rohan R. Vakhariya ¹, Swati S. Talokar ¹, V. R. Salunkhe ¹, C. S. Magdum ¹

Affiliations
1 Rajarambapu College of Pharmacy, Kasegaon, Tal. Walwa, Dist. Sangli, Maharashtra, IN

Abstract

The present research work support preformulation, formulation, development and optimization of solid lipid nanoparticles containing simvastatin as an antihyperlipidemic drug. The different batches of formulation were obtained using simvastatin as active pharmaceutical ingredient, stearic acid as the source of lipid and β - cyclodextrin as a polymer. Solid lipid nanoparticles of simvastatin were prepared by precipitation method. The prepared formulations were evaluated for various parameters like Particle size, Percentage entrapment efficiency, Measurement of Zeta Potential, FTIR, in-vitro release study. The result was found to be within standard limit. FTIR study reports indicated that there was no interaction between Simvastatin and other excipients. Motic microscope shown that the nanoparticles size ranges from 100-550nm. Percent entrapment efficiency was between 61.40% - 92.30%. Based on the resultants of the entrapment and particle size of nanoparticles, formulation S6 was observed to be optimized formulation. The optimized formulation exhibited 63.75% cumulative drug release after 24 h. The optimized batch shows mean of zeta potential at -44.0 mV.

Keywords

Antihyperlipidemic Drug, β–Cyclodextrin, Entrapment Efficiency, Zeta Potential, Motic Microscope.

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Rohan R. Vakhariya ¹, Swati S. Talokar ¹, V. R. Salunkhe ¹, C. S. Magdum ¹

Affiliations
1 Rajarambapu College of Pharmacy, Kasegaon, Tal-Walwa, Dist-Sangli, Maharashtra, IN

Abstract

People with mild cognitive impairment have problems with memory, language, thinking or judgment that are greater than the cognitive changes associated with normal aging. Cognitive disorders can be objectively measured in cognitive tests, but the changes in cognitive abilities are usually not serious enough to interfere with daily life and independence. A person with cognitive impairment has an increased risk of developing Delirium, Dementia Alzheimer's disease and Amnesia, but some people with cognitive impairment remain stable and others show improved cognitive abilities over time. There is no proper cure for the cognitive disorders and much of the treatments available have been able to only delay the progression of the disorders or provide symptomatic relief for a short time period. Therefore there is need for a different approach to the treatment of these cognitive disorders. Different medicinal plants have been used since ancient times in the treatment of various cognitive disorders. Therefore pharmacological screening of medicinal plants may provide useful leads in the discovery of new drugs for the treatment of cognitive disorders. Variety of medicinal plants which can be used in cognitive enhancement is reviewed in this article. Phytochemical substances such as alkaloids, curcuminoids, triterpenoids and withenolides with pharmacological activities related to cognitive disorders treatment are discussed in this review.

Keywords

Cognitive Disorders, Alzheimer’s Disease, Medicinal Plants, Cognitive Enhancement, Phytochemical Substances.

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Sachin U. Rakesh ¹, V. R. Salunkhe ¹, P. N. Dhabale ¹, K. B. Burade ¹

Affiliations
1 Govt. College of Pharmacy, Vidyanagar, Tal. Karad- 415 124, Dist. Satara, IN

Abstract

A sensitive and reliable high performance thin layer chromatographic method has been developed for quantitation of gallic acid in the dried flowers of Nymphaea stellata willd. The hydroalcoholic extract of N. stellata was chromatographed on silica gel 60 F254 plates with chloroform: ethyl acetate: formic acid, 7.5: 6: 0.5 (v/v/v), as mobile phase. Detection and quantitation were performed by densitrometric scanning at = 292 nm, by using deuterium lamp. The accuracy of the method was checked by conducting recovery studies at three different levels, using the standard addition method. The average recovery of gallic acid was found to be 98.33%. The proposed HPTLC method provide a good resolution of gallic acid from other constituents present in hydroalcoholic extract of dried flowers of N. stellata and can be used for quantitation of gallic acid present in extract. The method is rapid, simple and precise.

Keywords

HPTLC, Gallic Acid, Nymphaea stellata Willd.

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